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br Disclosure statement br Acknowledgments We would like to
2018-11-12
Disclosure statement Acknowledgments We would like to express our sincere gratitude to our collaborators from Cellular Dynamics International Inc., especially to Susan DeLaura, Lucas Chase, Jeff Grinager, David Majewski and Monica Strathman for the support on the preparation of manuscript. We
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OCT NANOG and THY are referred
2018-11-12
OCT4, NANOG, and THY1 are referred to as stem cell pluripotency markers. NANOG is expressed in porcine male germ cells, but OCT4 is not expressed in gonocytes of neonatal testes (Goel et al., 2008). However, OCT4 expression is detected in 2week cultures of PGP9.5 positive porcine spermatogonia from
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hiv integrase inhibitor br Materials and methods br Acknowle
2018-11-12
Materials and methods Acknowledgements We would like to thank Dr. Keisuke Okita and Prof. Shinya Yamanaka for providing the plasmids. Furthermore, we would like to thank Prof. Duncan R. Smith for proof reading the article. This work was supported by the Thailand Research Fund (MRG5980187), Nat
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br Materials and methods br Results br Discussion Human
2018-11-12
Materials and methods Results Discussion Human olfactory stem cells can be grown in neurospheres (Reynolds and Weiss, 1992). Murrell et al. obtained human mucosa by biopsy and generated OSs. They separated olfactory mucosa into lamina propria and epithelium. After separately dissociated, ce
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The occurrence of airway epithelial thickening in
2018-11-12
The occurrence of airway epithelial thickening in egfr inhibitor leads to a decrease in airway lumen size, consequently resulting in increased airway resistance corresponding to AHR (Elias, Zhu, Chupp, and Homer, 1999). Data from pediatric and non-fatal asthma studies have shown epithelial thicknes
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Q-VD(OMe)-OPh br Immunofluorescence based detection of prote
2018-11-12
Immunofluorescence-based detection of proteins The cells were fixed with 4% paraformaldehyde for 20min at RT and washed two times with PBS. The immunofluorescence-based staining of proteins was carried out as described in Matz and Adjaye (2015). Primary Q-VD(OMe)-OPh were used as follows: OCT4 (
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Induced pluripotent stem iPS cells derived from human somati
2018-11-09
Induced pluripotent stem (iPS) casein kinase derived from human somatic cells by expression of defined transcription factors represent a powerful novel system for disease modeling (Park et al., 2008; Takahashi et al., 2007). Moreover, the recent development of highly efficient genome editing tools
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br Experimental Procedures For detailed experimental procedu
2018-11-09
Experimental Procedures For detailed experimental procedures, see the Supplemental Experimental Procedures. Author Contributions Acknowledgments Introduction Cell transplantation in the central nervous system (CNS) requires exogenous cells to survive and integrate into the neural circui
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pkc inhibitors We previously discovered that ATM in addition
2018-11-09
We previously discovered that ATM, in addition to serving many roles in cell-cycle regulation and DNA damage response, also functions as a key epigenetic regulator in neurons, likely underlying the ataxic symptoms as well as neurodegeneration. ATM indirectly regulates nuclear-cytoplasmic shuttling o
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An approximate solution is sought
2018-11-09
An approximate solution is sought in the form of an output of an artificial neural network of the given architecture: whose weights are determined when minimizing the error functional and for our case, F(x, y, α) = –α(y – cosx). Test points () are chosen to be random and distributed uniformly
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For example if f h
2018-11-09
For example, if f(h)=h, then (this is a rough estimate). We should note here that Varin announced an unprecedented exact value of α=0.33205… (by 32 digits!), with an unlimited accuracy [1,3] (see also a brief summary in Ref. [4]). Aside from the above-described, the Pade approximation [5] is appli
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daunorubicin The molecular dynamic investigation of the diff
2018-11-09
The molecular dynamic investigation of the daunorubicin characteristics in nanocrystalline materials through the sum of squared atomic displacements has been successfully used by us in the studies in grain boundary self-diffusion in the high-purity copper and the diffusion of copper along the grain
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br Methods br Results The analyses indicate how microscale p
2018-11-09
Methods Results The analyses indicate how microscale pedestrian environment features varied by block group median income and race/ethnicity (Table 2). In all three regions, the findings revealed that mdm2 inhibitor certain microscale pedestrian features were less favorable (i.e., lower qualit
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br Conclusions br Outstanding Questions br Search
2018-11-09
Conclusions Outstanding Questions Search strategy and Selection Criteria The following are the supplementary data related to this article. Authors\' Contributions Competing Interests Introduction Dendritic cells (DCs) are professional antigen presenting cells (APCs) that can effic
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Finally we found that macroscopic analysis
2018-11-09
Finally, we found that macroscopic analysis of whole tumours could predict response, and baseline Ktrans was the strongest predictor, which suggests VEGF is main determinant of vascular leakiness, though not necessarily angiogenesis. Although baseline gene expression did not strongly correlate with
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